Abstract
Ancient DNA was analyzed in altogether 28 Late Eneolithic and Bronze Age human skeletons form 4 sites in southern Ukraine. More than 0,3% of human DNA was preserved only in 13 skeletons. The results of our analyses provide evidence that recovery of DNA molecules suitable for genetic analyses is more dependent on the specificity of the archaeological site and is not strongly correlated with particular environmental factors.
References
Allentoft M.E., Collins M., Harker D., Haile J., Oskam C.L., Hale M.L. 2012. The half-life of DNA in bone: measuring decay kinetics in 158 dated fossils. Proceedings of the Royal Society B: Biological Sciences B 279 (1748): 4724-4733.
Geigl E.M. 2002. On the circumstances surrounding the preservation and analysis of very old DNA. Archaeometry 44 (3): 337-342.
Goslar T., Klochko V.I., Kośko A., Włodarczak P., Żurkiewicz D. 2015. Chronometry of Late Eneolithic and ‘Early Bronze’ Cultures in the Middle Dniester Area: Investigations of the Yampil Barrow Complex. Baltic-Pontic Studies 20: 257-292.
Günther T., Valdiosera C., Malmström H., Urena I., Rodriguez-Varela R., Sverrisdóttir Ó.O. 2015. Ancient genomes link early farmers from Atapuerca in Spain to modernday Basques. Proceeding of the National Academy of Sciences of the United States of America 112 (38): 11917-11922.
Hagelberg E., Bell L.S. Allen T., Boyde A., Jones S.J., Clegg J.B. 1991. Analysis of ancient bone DNA: techniques and applications. 3 Philosophical Transactions: Biological Sciences 33 (1268): 399-407.
Hansen H.B., Damgaard P.B., Margaryan A., Stenderup J., Lynnerup N., Willerslev E. 2017. Comparing Ancient DNA Preservation in petrous bone and tooth cementum. PLOS ONE 12 (1): e0170940.
Jankowski M., Dąbrowski M., Jasińska J., Michalski A. 2017. Yampil Barrow Complex: Prydnistryanske 1 and Klembivka 1. Pedological-environmental aspects of location. Baltic-Pontic Studies 22: 41-53.
Juras A., Chyleński M., Krenz-Niedbała M., Malmström H., Ehler E., Pospieszny Ł. 2017. Investigating kinship of Neolithic post-LBK human remains from Krusza Zamkowa, Poland using ancient DNA. Forensic Science International: Genetics 26: 30-39.
Juras A., Chyleński M., Ehler E., Malmström H., Żurkiewicz D., Włodarczak P., Wilk S., Peška J., Fojtík P., Králík M., Libera J., Bagińska J., Tunia K., Klochko V., Dabert M., Jakobsson M., Kośko A. forthcoming. Ancient mitochondrial genome data support the participation of females in the Yamnaya migration into Central Europe.
Litvinova L.V., Łukasik S., Żurkiewicz D., Gwizdała M., Chyleński M., Malmström H, 2015. Anthropological Description of Skeletal Material from the Dniester Barrow- Cemetery Complex, Yampil Region, Vinnitsa Oblast (Ukraine). Baltic-Pontic Studies 20: 293-336.
Meyer M., Kircher M. 2010. Illumina sequencing library preparation for highly multiplexed target capture and sequencing. Cold Spring Harbor Protocols 6: pdb.prot5448.
Pinhasi R., Fernandes D., Sirak K., Novak M,. Connell S., Alpaslan-Roodenberg S. 2015. Optimal ancient DNA Yields from the inner ear part of the human petrous bone. PLOS ONE. 10 (6): e0129102.
Pruvost M., Schwarz R., Correia V.B., Champlot S., Braguier S., Morel N. 2007. Freshly excavated fossil bones are best for amplification of ancient DNA. Proceeding of the National Academy of Sciences of the United States of America 104 (3): 739.
Schotsmans E.M.J., Májrquez-Grant N., Forbes S.L. (Eds). 2017. Taphonomy of human remains: forensic analysis of the dead and the depositional environment. West Sussex.
Schwarz C., Debruyne R., Kuch M., McNally E., Schwarcz H., Aubrey A.D. 2009. New insights from old bones: DNA preservation and degradation in permafrost preserved mammoth remains. Nucleic Acids Research 37 (10): 3215-3229.
Smith C.I., Chamberlain A.T., Riley M.S., Cooper A., Stringer C.B., Collins M.J. 2001. Neanderthal DNA: Not just old but old and cold? Nature 410 (6830): 771-772.
License
Copyright (c) 2017 Maciej Chyleński, Anna Juras, Danuta Żurkiewicz, Michał Jankowski, Piotr Włodarczak
![Creative Commons License](http://i.creativecommons.org/l/by-nc-nd/3.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 Unported License.