Keywords
AFLP markers
genetic diversity
evolution
How to Cite
Number of views: 81
Number of downloads: 43
Abstract
The genus Lolium is represented by selfpollinated L. loliaceum, L. persicum, L. remotum and L. temulentum and outpollinated L. multiflorum, L. perenne and L. rigidum. The aim of the present study was the analysis of phylogenetic relationships between all Lolium species on the base of AFLP markers. All Lolium species were analysed with 8 pairs of AFLP primers. Each pair of primers resulted in the amplification of large number of AFLP fragments. Most fragments were polymorphic and many of them were restricted to a single species. Only 10 fragments were monomorphic across all species tested. The UPGMA clustering grouped all species into two groups. The first group was represented by three selfpollinated species, L. persicum, L. remotum and L. temulentum while the second one is formed by three outpollinated species, i.e. L. multiflorum, L. perenne and L. rigidum. Out of these species, L. multiflorum and L. perenne are joined with the highest genetic similarity providing further credence to our earlier conclusion that both taxa can not be regarded as biological species. The grouping of L. loliaceum within outpollinated species may suggest that this taxon evolved within a group of outpollinated taxa, followed by several point mutations responsible for self-fertility.
Funding
This work and Luis Cabrita fellowship were supported by the European Community Grant: Marie Curie Host Fellowships for the Transfer of Knowledge, GenCrop. Contract No MTKD-CT-2004-509834.
References
BULIŃSKA-RADOMSKA Z. 1985. Relationships between five Lolium species (Poaceae). Pl Syst Evol 159: 217-227. DOI: https://doi.org/10.1007/BF00935973
CHARMET G. & BALFOURIER F. 1994. Isoenzyme variation and species relationships in the genus Lolium L. (ryegrasses, Graminaceae). Theor Appl Genet 87: 641-649. DOI: https://doi.org/10.1007/BF00222888
LOOS B. P. 1993a. Morphological variation in Lolium (Poaceae) as a measure of species relationships. Pl Syst Evol 188: 87-99. DOI: https://doi.org/10.1007/BF00937838
LOOS B. P. 1993b. Allozyme variation within and between populations in Lolium (Poaceae). Pl Syst Evol 188: 101-113. DOI: https://doi.org/10.1007/BF00937839
MURRAY M. & THOMPSON W. 1980. The isolation of high molecular weight plant DNA. Nucleic Acids Res. 8: 4321-4325. DOI: https://doi.org/10.1093/nar/8.19.4321
NEI M. & LI W. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76: 5269-5273. DOI: https://doi.org/10.1073/pnas.76.10.5269
POLOK K. 2005. Evolutionary status of closely related Lolium taxa. In: W. PRUS-GŁOWACKI & E. PAWLACZYK (eds.). Variability and Evolution - New Perspectives, seria Biologia 72: 195-208. UAM Poznań.
VOS P., HOGERS R., BLEEKER M., REIJANS M., VANDELEE T., HORNES M., FRIJTERS A., POT J., PELEMAN J., KUIPER M. & ZABEAU M. 1995. AFLP - a new technique for DNA fingerprinting. Nucl. Acids Res. 23: 4407-4414. DOI: https://doi.org/10.1093/nar/23.21.4407
ZIELIŃSKI R. & POLOK K. 2005. Molecular evolution and taxonomy of plants. In: W. PRUS-GŁOWACKI & E. PAWLACZYK (eds.). Variability and Evolution n New Perspectives, seria Biologia 72: 37-56. UAM Poznań.
ZIELIŃSKI R., POLOK K. & WÓJCIK-PATER I. 1997. Application of isoenzymes and RAPD-PCR in studies on genetic similarity of Lolium perenne (L.) and L. multiflorum (Lamp.). In: Z. STASZEWSKI, W. MŁYNIEC & R. OSIŃSKI (eds.). Ecological aspects of breeding fodder crops and amenity grasses, pp. 370-376. Proc. Of the 20th Meeding of Eucarpia Fodder Crops and Amenity Grasses Section. Radzików, Poland, 7-10 October 1996.
License
Copyright © by Adam Mickiewicz University, Poznań, Poland, Department of Plant Taxonomy, 2006OPEN ACCESS