Infrageneric reclassification and phylogenetic inference in the genus Masdevallia Ruiz & Pav. (Orchidaceae, Pleurothallidinae) n preliminary results of nrDNA based analysis
Journal cover Biodiversity: Research and Conservation, no. 3-4, year 2006
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Keywords

Orchidaceae
Pleurothallidinae
Masdevallia
ITS
infrageneric classification
maximum parsimony (MP)

How to Cite

Matuszkiewicz, A., & Tukałło, P. (2006). Infrageneric reclassification and phylogenetic inference in the genus Masdevallia Ruiz & Pav. (Orchidaceae, Pleurothallidinae) n preliminary results of nrDNA based analysis. Biodiversity: Research and Conservation, (3-4), 213–219. https://doi.org/10.14746/biorc.2006.3-4.4

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Abstract

The genus Masdevallia is one of the largest genera within the subtribe Pleurothallidinae. Its present infrageneric classification, based on easily recognized morphological features, does not reflect natural relationships and evolutionary trends within the genus. To test its relative utility in infrageneric classification and in resolving genus phylogeny, sequences of the Internal Transcribed Spacer region (ITS1-5,8S-ITS2) were identified for 37 taxa and combined with additional 35 sequences taken from the GenBank. Results of maximum parsimony based on phylogenetic analysis indicate that the level of sequence divergence is sufficient to resolve infrageneric relationships. The implications of current results for the classification of Masdevallia are also briefly discussed. Many of the infrageneric changes made by C.A. Luer since his first classification of the genus in 1986 are not supported by results of this study.

https://doi.org/10.14746/biorc.2006.3-4.4
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Funding

Scientific work financed from the resources earmarked for science in years 2005-2008 as the Research Project no. 2 P04C 074 27.

References

BALDWIN B. G. 1992. Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: en example from the Compositae. Molecular Phylogenetics Evolution 1: 3-16. DOI: https://doi.org/10.1016/1055-7903(92)90030-K

BERG VAN DEN C., HIGGINS W. E., DRESSLER R. L., WHITTEN W. M., SOTO-ARENAS M. A., CULHAM A. & CHASE M. W. 2000. A phylogenetic analysis of Laeliinae (Orchidaceae) based on sequence data from internal transcribed spacers (ITS) of nuclear ribosomal DNA. Lindleyana 15(2): 96-114.

BODO SLOTTA T. A. 2000. Phylogenetic Analysis of Iliamna (Malvaceae) Using the Internal Transcribed Spacer Region. PhD Thesis, Faculty of the Virginia Polytechnic Institute and State University, Blackburg, USA.

CAMERON K. M. & CHASE M. W. 1999. Phylogenetic relationships of Pogoniinae (Vanilloideae, Orchidaceae): An herbaceous example of the Eastern North America - Eastern Asia phytogeographic disjunction. Journal of Plant Research 112: 317-329. DOI: https://doi.org/10.1007/PL00013873

COX A. V., PRIDGEON A. M., ALBERT V. A. & CHASE M. W. 1997. Phylogenetics of the slipper orchids (Cypripedioideae, Orchidaceae): nuclear rDNA ITS sequences. Plant Systematic and Evolution 208: 197-223. DOI: https://doi.org/10.1007/BF00985442

DOUZERY E. J. P., PRIDGEON A. M., KORES P., LINDER P., KURZWEIL H. & CHASE M. W. 1999. Molecular phylogenetics of Diseae (Orchidaceae): a contribution from nuclear ribosomal ITS sequences. Am. J. Bot. 86: 887-899. DOI: https://doi.org/10.2307/2656709

FELSENSTEIN J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783-791. DOI: https://doi.org/10.1111/j.1558-5646.1985.tb00420.x

GRAVENDEEL B., CHASE M. W., VOGEL E. F., ROOS M. C., MES H. M. & BACHMANN K. 2001. Molecular phylogeny of Coelogyne (Epidendroideae; Orchidaceae) based on plastid RFLPS, matK and nuclear ribosomal ITS sequences: evidence for polyphyly. Am. J. Bot. 88(10): 1915-1927. DOI: https://doi.org/10.2307/3558367

GIELLY L., YUAN Y., M., KUPFER P. & TABERLET P. 1996. Genetic use of noncoding regions in the genus Gentiana L.: Chloroplast trnL (UAA) intron versus Nuclear Ribosomal Internal Transcribed Spacer sequences. Molecular Phylogenetics and Evolution 5(3): 460-466. DOI: https://doi.org/10.1006/mpev.1996.0042

HOLMQUIST R. 1983. Transitions and transversions in evolutionary descent: an approach to understanding. Journal of Molecular Evolution 19: 134-144. DOI: https://doi.org/10.1007/BF02300751

KRÄNZLIN F. 1925. Monographie der Gattungen Masdevallia, Lothiania, Scaphosepalum, Cryptophoranthus & Pseudoctomeria. Repert. Spec. Nov. Regni Veg. Beih. 34: 1-240.

LAWRENCE A. A. & CAMPBELL CH. S. 1999. Phylogeny of Rubus (Rosaceae) based on nuclear ribosomal DNA internal transcribed spacer region sequences. Am. J. Bot. 86(1): 81-97. DOI: https://doi.org/10.2307/2656957

LUER C. A. 1986a. Icones Pleurothallidinarum I. Systematics of Pleurothallidinae (Orchidaceae). Monographs in Systematic Botany, vol. 15. Missouri Botanical Garden Press, St. Louis, Missouri.

LUER C. A. 1986b. Icones Pleurothallidinarum II: Systematics of Masdevallia (Orchidaceae). Monographs in Systematic Botany, vol. 16. Missouri Botanical Garden Press, St. Louis, Missouri.

LUER C. A. 2000a. Icones Pleurothallidinarum XIX: Systematics of Masdevallia Part One. Monographs in Systematic Botany, vol. 77. Missouri Botanical Garden Press, St. Louis, Missouri.

LUER C. A. 2000b. Icones Pleurothallidinarum XXI: Systematics of Masdevallia, Part Two. Monographs in Systematic Botany, vol. 82. Missouri Botanical Garden Press, St. Louis, Missouri.

LUER C. A. 2001. Icones Pleurothallidinarum XXII: Systematics of Masdevallia, Part Three. Monographs in Systematic Botany, vol. 86. Missouri Botanical Garden Press, St. Louis, Missouri.

LUER C. A. 2002. Icones Pleurothallidinarum XXIII: Systematics of Masdevallia, Part Four. Monographs in Systematic Botany, vol. 87. Missouri Botanical Garden Press, St. Louis, Missouri.

LUER C. A. 2003. Icones Pleurothallidinarum XXV: Systematics of Masdevallia, Part Five. Monographs in Systematic Botany, vol. 91. Missouri Botanical Garden Press, St. Louis, Missouri.

MATSUMOTO S., KOUCHIB M., YABUKIB Y., KUSUNOKIA M., UEDAC Y. & FUKUIB H. 1998. Phylogenetic analyses of the genus Rosa using the matK sequence: molecular evidence for the narrow genetic background of modern roses. Scientia Horticulturae 77: 73-82. DOI: https://doi.org/10.1016/S0304-4238(98)00169-1

PRIDGEON A. M. & CHASE M. W. 2001. A phylogenetic reclassification of Pleurothallidinae (Orchidaceae). Lindleyana 16: 235-271.

PRIDGEON A. M., CRIBB P. J., CHASE M. W. & RASMUSSEN F. N. 2005. Genera Orchidacearum: Volume 4 - Epidendroideae (Part One). Oxford University Press, USA. DOI: https://doi.org/10.1093/oso/9780198507123.001.0001

PRIDGEON A. M., SOLANO R. & CHASE M. W. 2001. Phylogenetic relationships in Pleurothallidinae (Orchidaceae): combined evidence from nuclear and plastid DNA sequences. Am. J. Bot. 88: 2286-2308. DOI: https://doi.org/10.2307/3558390

REICHENBACH H. G. 1861. Walpers Annales. Ann. Bot. Syst. 6: 188-195. DOI: https://doi.org/10.1002/andp.18611880312

RYAN A., WHITTEN W. M., JOHNSON M. A. T. & CHASE M. W. 2000. A phylogenetic assessment on Lycaste and Anguloa (Orchidaceae: Maxillarieae). Lindleyana 15(1): 35-45.

SHAW J., LICKEY E. B., BECK J. T., FARMER S. B., LIU W., MILLER J., SIRIPUN KUNSIRI C., WINDER C. T., SCHILLING E. E. & SMALL R. L. 2005. The tortoise and the hare II: relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis. Am. J. Bot. 92: 142-166. DOI: https://doi.org/10.3732/ajb.92.1.142

SIMMONS M. P. & OCHOTERENA H. 2000. Gaps as characters in sequence-based phylogenetic analyses. Syst. Biol. 49: 369-381. DOI: https://doi.org/10.1093/sysbio/49.2.369

SMALL R. L. & WENDEL J. F. 2000. Phylogeny, duplication, and intraspecific variation of Adh sequences in New World diploid cottons (Gossypium L., Malvaceae). Molecular Phylogenetics and Evolution 16: 73-84. DOI: https://doi.org/10.1006/mpev.1999.0750

SWOFFORD D. L. 2000. PAUP*: Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4.0b2. Sinauer Associates, Sunderland MA.

WENDEL J. F. & DOYLE J. J. 1998. Phylogenetic incongruence: window into genome history and molecular evolution. In: D. E. SOLTIS, P. S. SOLTIS & J. J. DOYLE (eds.), Molecular Systematics of Plants II: DNA sequencing, pp. 265-296. Kluwer Academic Publishers, Boston. DOI: https://doi.org/10.1007/978-1-4615-5419-6_10

WHITE T. J., BRUNS T., LEE S. & TAYLOR J. W. 1990. Amplification and direct sequencing of fungal ribosomal DNA genes for phylogenetics. In: M. A. INNIS, D. H. GELGARD, J. J. SNINSKY & T. J. WHITE (eds.). Protocols: A Guide to Methods and Application, pp. 315-322. Academic Press, New York. DOI: https://doi.org/10.1016/B978-0-12-372180-8.50042-1

WOOLWARD F. H. 1896. Genus Masdevallia. Issued by the Marquess of Lothian, K.T., chiefly from plants in his collection of orchids at Newbattle abbey; plates and descriptions by Miss Florence H. Woolward; with additional notes by F.C. Lehmann. DOI: https://doi.org/10.5962/bhl.title.46205

YUAN Y. M. & KUPFER PH. 1997. The monophyly and rapid evolution of Gentiana sect. Chondrophyllae Bunge s.l. (Gentianaceae): evidence from the nucleotide sequences of the internal transcribed spacers of nuclear ribosomal DNA. J. Linn. Soc. Bot. 123: 25-43. DOI: https://doi.org/10.1111/j.1095-8339.1997.tb01403.x