Abstract
Nine species of the genus Calypogeia Raddi are currently known from Europe: C. azurea, C. integristipula, C. neesiana,C. suecica, C. muelleriana, C. sphagnicola, C. fissa, C. arguta, and C. azorica. Recently, another species, morphologically resembling C. muelleriana but genetically distinct from it, was detected using isozyme markers. In the present study, relationships between the newly detected species (C. sp. nov.) and typical C. muelleriana were analyzed using the DNA sequencesdata of three regions from the chloroplast genome: introns of trnG and trnL genes and intergenic spacer trnH-psbA. Calypogeia sp. nov. differs from C. muelleriana s. str. (typical form) in all examined chloroplast regions. It differs as well from C. azurea, which was used as a reference species. The number of fixed nucleotide differences between C. muelleriana s. str. and C. sp. nov. is almost the same as between C. muelleriana s. str. and C. azurea. The results of the present study suggest a closer affinity of C. sp. nov. to C. azurea than to C. muelleriana s. str. in Europe, C. muelleriana s. str. was noted in Poland, Germany, Holland, United Kingdom and Azores. Samples determined as C. sp. nov., besides Poland, were so far detected also in North America
References
Bischler H. 1963. The genus Calypogeia Raddi in Central and South America. I-III. Candollea 18: 19-128.
Bischler H. & Boisselier-Dubayle M. C. 2000. New approaches to the systematics of liverworts. Nova Hedwigia 70: 37-44.
Buczkowska K. 2004a. Genetic differentiation of Calypogeia fissa Raddi (Hepaticae Jungermanniales) in Poland. Pl. Syst. Evol. 247: 187-201.
Buczkowska K. 2004b. The genus Calypogeia Raddi (Hepaticae Jungermnniales) in Poland biometrical analysis of morphological and anatomical variation. Nova Hedwigia 78: 121-146.
Buczkowska K. 2010. Morphological differentiation of Calypogeia muelleriana (Jungermanniales, Hepaticae) in Poland. Biodiv. Res. Conserv. 17: 23-32.
Buczkowska K., Adamczak M., Bączkiewicz A., Chudzińska E. & Wachowiak W. 2006. In vitro propagation of cryptic species of Aneura pinguis (Metzgeriales, Hepaticae). Cryptogamie Bryol. 27: 241-251.
Buczkowska K. & Bączkiewicz A. 2011. New taxon of the genus Calypogeia (Jungermanniales, Hepaticae) in Poland. Acta Soc. Bot. Pol. 80: 327-333.
Buczkowska K. & Dabert M. 2011. The development of species-specific SCAR markers for delimitation of Calypogeia species. J. Bryol. 33: 291-299.
Buczkowska K., Sawicki J., Szczecińska M., Rosadziński S., Rabska M., Bączkiewicz A. 2011. Two morphologically distinct groups of the Calypogeia fissa complex were found in Europe. Biodiv. Res. Conserv. 23: 7-19.
Buczkowska K., Odrzykoski I. J. & Chudzińska E. 2004. Delimitation of some European species of Calypogeia Raddi (Jungermnniales, Hepaticae) based on cytological characters and multienzyme phenotype. Nova Hedwigia 78: 147-163.
Buczkowska K., Sawicki J., Szczecińska M., Klama H. & Bączkiewicz A. 2012a. Allopoliploid speciation of Calypogeia sphagnicola (Jungermanniopsiada, Calypogeiaceae) based on isozyme and DNA markers. Pl. Syst. Evol. 298: 549-560.
Buczkowska K., Sawicki J., Szczecińska M., Klama H. & Bączkiewicz A. 2012b. Isozyme and DNA markers reveal a new genetically distinct taxon of Calypogeia sphagnicola (Jungermanniopsida, Calypogeiaceae). Pol. Bot. J. 57: 95-107.
Buczkowska K., Gonera P. & Hornik B. 2012c. PCR-based molecular markers for identification of taxa from the Calypogeia fissa complex (Jungermanniopsida, Calypogeiaceae). Biodiv. Res. Conserv. 28: 9-18.
Celka Z., Szczecińska M. & Sawicki J. 2010. Genetic relationship between some of Malva species as determined with ISSR and ISJ markers. Biodiv. Res. Conserv. 19: 23-32.
Celka Z., Szczecińska M., Sawicki J. & Shevera M. V. 2012. Molecular studies did not support the distinctiveness of Malva alcea and M. excisa (Malvaceae) in Central and Eastern Europe. Biologia 67: 1088-1098. DOI: 10.2478/s11756-012-0107-9
Damsholt K. 2002. Illustrated Flora of Nordic Liverworts and Hornworts. 456-476 pp. Nordic Bryological Society. Lund.
Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783-791.
Fuselier L., Davison P. G., Clements M., Shaw B., Devos N., Heinrichs J., Hentschel J., Sabovljevic M., Szovenyi P., Schuette S., Hofb auer W & Shaw A. J. 2009. Phylogeographic analysis reveal distinct lineages of the liverworts Metzgeria furcata (L.) Dumort. and Metzgeria conjugata Lindb. (Metzgeriaceae) in Europe and North America. Biol. J. Linn. Soc. 98: 745-756.
Górniak M., Mytnik-Ejsmont J., Rutkowski P., Tukałło P., Minasiewicz J & Szlachetko D. L. 2006. Phylogenetic relationships within the subtribe Spiranthinae s.l. (Orchidaceae) inferred from the nuclear ITS region. Biodiv. Res. Conserv. 1-2: 18-24.
Grądzielewska A. 2011. Application of the ISSR metod to estimate the genetic similarity of Dasypyrum villosum (L.) P. Candargy Greek populations to Triticum and Secale species. Biodiv. Res. Conserv. 21: 7-12. DOI 10.2478/v10119-011-0002-1
Hassel K. & Gunnarsson U. 2003. The use of inter simple sequence repeats (ISSR) in bryophyte population studies. Lindbergia 28: 152-157.
Hey J. & Wakeley J. 1997. A coalescent estimator of the population recombination rate. Genetics 145: 833-846.
Heinrichs J., Hentschel J., Bombosch A., Fiebig A., Reise J., Edelmann M., Kreier H. P., Schäfer-Verwimp A., Caspari S., Schmidt A. R., Zhu R. L., Konrat M., Shaw B. & Shaw A. J. 2010. One species or at least eight? Delimitation and distribution of Frullania tamarisci (L) Dumort s l (Jungermanniopsida Porellales) inferred from nuclear and chloroplast DNA markers. Molec. Phylog. Evol. 56: 1105-1114.
Jankowiak K. & Szwekowska-Kulińska Z. 2004. Organellar inheritance in the allopolyploid liverwort species Porella baueri (Porellaceae): reconstructing historical events using DNA sequence analysis. Monographs in Systematic Botany from the Missouri Botanical Garden 98: 404-414.
Krawczyk K., Szczecińska M. & Sawicki J. 2013. Evaluation of 11 single-locus and seven multilocus DNA barcodes in Lamium L. (Lamiaceae). Molec. Ecol. Res. DOI: 10.1111/1755-0998.12175
Kreier H. P., Feldberg K., Mahr F., Bombosch A., Schmidt A. R., Zhu R. L., Konrat M., Shaw B., Shaw A. J. & Heinrichs J. 2010. Phylogeny of the leafy liverwort Ptilidium: Cryptic speciation and shared haplotypes between Northern and Southern Hemispheres. Molec. Phylog. Evol. 57: 1260-1267.
Murray M. & Thompson W. F. 1980. Rapid isolation of molecular weight plant DNA. Nucleic Acids Res. 8: 4321-4325.
Müller K. 1951-1958. Die Lebermoose Europas. In: Dr. L. Rabenhorst’s Kryptogamen Flora von Deutschland, Österreich und der Schweiz. Akademische Verlagsgesellschaft Geest & Portig K-G, Leipzig. Pacak A. & Szweykowska-Kulińska Z. 2003. Organellar inheritance in liverworts. An example of Pellia borealis. J. Mol. Evol. 56: 11-17.
Plášek V., Sawicki J., Marková I. & Wierzcholska S. 2011.
Orthotrichum affine var. bohemicum (Orthotrichaceae), a new variety of epiphytic moss from the Czech Republic. Acta Soc. Bot. Pol. 80(4): 335-340.
Sang T., Crawford D. J. & Stuessy T. F. 1997. Chloroplast DNA phylogeny reticulate evolution and biogeography of Paeonia (Paeoniaceae). Am. J. Bot. 84: 1120-1136.
Sawicki J., Plášek V. & Szczecińska M. 2010. Molecular studies resolve Nyholmiella (Orthotrichaceae) as a separate genus. J. Syst. Evol. 48: 183-194.
Sawicki J. & Szczecińska M. 2007. Semi-specific intron-exon splice junction markers in bryophyte studies. Biodiv. Res. Conserv. 5-8: 25-30.
Schumacker R. & Váňa J. 2005. Identification keys to the liverworts and hornworts of Europe and Macaronesia (Distribution and status). 209 pp. Sorus, Poznań.
Schuster R. M. 1969. The Hepaticae and Anthocerotae of North America east of the hundredth meridian, vol. 2. Columbia University Press, New York-London.
Shaw A. J., Cox C. J. & Boles S. B. 2003. Polarity of peatmoss (Sphagnum) evolution: who says bryophytes have no roots? Am. J. Bot. 90: 1777-1787.
Shlyakov R. N. 1979. Pieczenocznyje mchi sjeviera S.S.S.R. t. 2 Lofoziewyje, (Hepatics of the northern territories of the USSR, vol. 2, Lophoziaceae). Nauka, Leningrad.
Singh A. P. & Nath V. 2007. A New Calypogeia Raddi from India. Taiwania 52: 320-323.
Söderström L., Urmi E. & Váňa J. 2002. Distribution of Hepaticaeand Anthocerotae in Europe and Macaronesia. Lindbergia 27: 3-47. Söderström L., Urmi E. & Váňa J. 2007. The distribution of Hepaticae and Anthocerotae in Europe and Macaronesia - Update 1-427. Cryptogamie Bryol. 28: 299-350.
Szweykowska-Kulińska Z., Pacak A. & Jankowiak K. 2002. New DNA markers for discrimination between closely-related species and for the reconstruction of historical events; an example using liverworts. Cell. Mol. Biol. Lett. 7: 403-416.
Szweykowski J. 1984. Species problems and taxomonic methods in Bryophytes in: R. M. Schuster The New Manual of Bryology. The Hattori Bot. Lab. Nichinan, Miyazaki, vol. 2, pp. 1130-1171.
Szweykowski J. 2006. An annotated checklist of Polish liverworts. In: Z. Mirek (ed.) Biodiversity of Poland, 4, pp. 18-20. W. Szafer Institute of Botany, Polish Academy of Sciences, Kraków.
Tamura K., Peterson D., Peterson N., Stecher G., Nei M. & Kumar S. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol. Biol. Evol. 28: 2731-2739.
Taberlet P., Coissac E., Pompanon F., Gielly L., Miquel C., Valentini A., Vermat T., Corthier G., Brochmann C. & Willerslev E. 2006. Power and limitations of the chloroplast trnL (UAA) intron for plant DNA barcoding. Nucleic Acids Res. 35: e14.
Wick ett N. J. & Goff inet B. 2008. Origin and relationships of the myco-heterotrophic liverwort Crypthotallus mirabilis Malmb. (Metzgeriales, Marchantiophyta). Bot. J. Linn. Soc. 156: 1-12.
Wilson R., Gradstein S.R., Schneider H. & Heinrichs J. 2007. Unravelling the phylogeny of Lejeuneaceae (Jungermanniopsida): evidence for four main lineages. Molec. Phylog. Evol. 43: 270-282.