Abstract
:Asian ginseng (Panax ginseng) and American ginseng (P. quinquefolius) are valuable medicinal herbs whose roots have been used for ages in traditional medicine in China and North America as vitalizing and stimulating agents. The roots are obtained mainly from field cultivation, which is a slow (5-7 years long), laborious, and troublesome process; so in vitro methods started to be used to produce ginseng biomass. In our study, non-organogenic callus of P. quinquefolius synthesized the same active substances like field roots, for more than 6 years. The ginsenosides are derivatives of protopanaxadiol (Rb1, Rb2, Rc, Rd) or protopanaxatriol (Rg1, Re). The synthesis of Rg1 and Re metabolites is preferred in calli cultured in the dark and with ageing of culture.
References
Choi K. T., Ahn I. O., Park J. S. 1994. Production of ginseng saponin in tissue culture of ginseng (Panax ginseng C.A. Meyer). Russian J. Plant Physiol. 41:784-788.
Ellis D. D., Zeldin E., Brodhagen M., Russin W. A., Mcc own B. H. 1996. Taxol production in nodule cultures of Taxus. J. Nat. Prod. 59: 246-250.
Hibb ino K., Ushiyama K. 1998. Commercial production of ginseng by plant cell culture technology.In: Plant Cell Culture for the production of Food Ingredients Proceedings of ACS symposium, (Fu Tj., Singh G., Curtis Wr., Eds.) April 13-17, San Francisco, CA, Plenum, New York.
Hikino H. 1991. Traditional remedies and modern assessment the case of ginseng. In: The medicinal plant industry. (Wijesekera R. O. B., Ed.), Chapter 11, p.149-162, CRC Press. Boca, Raton.
Huang T., Gao W. Y., Wang J., Cao Y., Zhao Y. X., Liu C. X. 2010. Selection and optimization of a high-producing tissue culture of Panax ginseng C. A. Meyer. Acta Physiol. Plant. 32: 765-772.
Jagetia C. G. 2007. Radioprotective Potential of Plants and Herbs against the Effects of Ionizing Radiation. J. Clin. Biochem. Nutr. 40: 74-81.
Karam S. N., Jawad F. M., Arikat N. A., Shibli R. A. 2003. Growth and rosmarinic acid accumulation in callus, cell suspension, and root cultures of wild Salvia fruticosa. Plant Cell Tissue and Organ. Cult. 73: 117-121.
Kim H. J., Kim M. H., Byon Y. Y., Park J. W., Jee Y., Joo H. G. 2008. Radioprotective effects of an acidic polysaccharide of Panax ginseng on bone marrow cells. J. Vet. Sci. 9: 359-365.
Kim J. H., Park C. Y., Lee S. J. 2006. Effects of sun ginseng on subjective quality of life in cancer patients: a double-blind, placebo-controlled pilot trial. J. Clin. Pharm. Ther. 31: 331-334.
Knöss W. 1995. Establishment of callus, cell suspension and shoot cultures of Leonurus cardiaca L. and diterpene analysis. Plant Cell Rep. 14: 790-793.
Langhansova L., Marsik P., Vanek T. 2005. Production of saponins from Panax ginseng suspension and adventitious root cultures. Biol. Plant. 49: 463-465.
Lee T. K., Johnke R. M., Allison R. R., O’brien K. F., Dobb s L. J. 2005. Radioprotective potential of ginseng. Mutagenesis 20: 237-243.
Li Q. F., Shi S. L., Liu Q. R., Tang J., Song J., Liang Y. 2008. Anticancer effects of ginsenoside Rg1, cinnamic acid, and tanshinone IIA in osteosarcoma MG-63 cells: nuclear matrix downregulation and cytoplasmic trafficking of nucleophosmin. Int. J. Biochem. Cell Biol. 40: 1918-1929.
Lim T. S., Na K., Choi E. M., Chung J. Y., Hwang J. K. 2004. Immunomodulating activities of polysaccharides isolated from Panax ginseng. J. Med. Food. 7: 1-6.
Mathur A., Mathur A. K., Gangw ar A. 2000. Saponin production by cell/callus cultures of Panax species. In: Saponins in Food, Feedstuff and Medicinal Plants (Oleszek W., Martson A., Ed.), Kluwer Academic Publishers, Dordrecht /Boston/London
Mathur A., Mathur A. K., Pal M., Uniyal G. C. 1999. Comparison of qualitative and quantitative in vitro ginsenoside production in callus cultures of three Panax species. Planta Med. 65: 484-486.
Morimoto S., Goto Y., Shoyama Y. 1994. Production of lithospermic acid B and rosmarinic acid in callus tissue and regenerated plantlets of Salvia miltiorrhiza. J. Nat. Prod. 57: 817-823.
Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
Nocerino E., Amato M., Izzo A. A. 2000. The aphrodisiac and adaptogenic properties of ginseng Fitoterapia 71, Supplement 1, 1 Aug, S1-S5.
Shim J. Y., Han Y., Ahn J. Y., Yun Y. S., Song J. Y. 2007. Chemoprotective and adjuvant effects of immunomodulator ginsan in cyclophosphamide-treated normal and tumor bearing mice. Int.J. Immunopathol. Pharmacol. 20: 487-97.
Wang C. Z., Aung H. H., Ni M., Wu J. A., Tong R., Wick s S., He T. C., Yuan C. S. 2007. Red American ginseng: ginsenoside constituents and antiproliferative activities of heat-processed Panaxquinquefolius roots. Planta Med. 73: 669-674.
Wang C. Z., Zhang B., Song. W. X., Wang A., Ni M., Luo X., Aung H. H., Xie J. T., Tong R., He T.C., Yuan C. S. 2006. Steamed American ginseng berry: ginsenoside analyses and anticancer activities. J. Agric. Food Chem. 54: 9936-9942.
Wang M., Larry J., Li G. J., Wu Y., Pang P., Tapan K., Basu Shan J. J. 2004. A proprietary extract from North American ginseng (Panax quinquefolium) enhances IL-2 and IFN-productions in murine spleen cells induced by Con-A. Int. Immunopharm. 4: 311-315.
Wang W., Rayburn E. R., Hao M., Zhao Y., Hill D. L., Zhang R., Wang H. 2008. Experimental therapy of prostate cancer with novel natural product anti-cancer ginsenosides. Prostate 1, 68: 809-819.
Wang X., Proctor J. T. A., Kakuda Y., Raj S. K., Saxena P. R. 1999. Ginsenosides in American ginseng: comparison of in vitro derived and field-grown plant tissues. J. Herbs, Spices & Medicinal Plans 6: 1-10.
Wu J., Zhong J. J. 1999. Production of ginseng and its bioactive components in plant culture: current technological and applied aspects. J. Biotechnol. 68: 89-99.
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