Abstract
There is a growing need for new and effective antimicrobial agents to treat life-threatening diseases. Fungal endophytes are receiving increasing attention by natural product chemists due to the diverse and structurally unprecedented compounds, which make them interesting candidates for drug discovery. The present study evaluates the antibacterial activity of ethyl acetate extract of the endophytic fungus Alternaria alternata VN3 on multi-resistant clinical strains of Staphylococcus aureus and Pseudomonas aeruginosa, as well as its cytotoxicity on MCF-7 and MDA MB-231 tumour cell lines of breast cancer. The maximum inhibition zone of 21.4±0.07 mm and 21.5±0.25 mm was observed for S. aureus strain 10 and P. aeruginosa strain 2, respectively. The ethyl acetate extract showed minimal inhibitory concentration ranging from 100 to 900 μg/ml for S. aureus and P. aeruginosa. Further, the ethyl acetate extract of A. alternata VN3 exhibited moderate anticancer activity against MCF-7 and MDA MB-231 cell lines. At 30 μg/ml the cell viability was decreased to 75.5% and 71.8% for MCF-7 and MDA MB-231 cells, respectively. These results clearly indicate that the metabolites of A. alternata VN3 are a potential source for production of new drugs.
References
Acar J. F, Goldstein F. W. 1991. Disk susceptibility test, Chapter 2. In: Antibiotics in laboratory medicine (Lorian V. MD, Ed.), pp. 17–52, 3rd ed., Williams & Wilkins, Maryland, USA.
Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403–410.
Al-Bayati F. A. 2008. Synergistic antibacterial activity between Thymus vulgaris and Pimpinella anisum essential oils and methanol extracts. J. Ethnopharmacol. 16: 403–406.
Arnold A. E., Maynard Z., Gilbert G., Coley P. D., Kursar T. A. 2000. Are tropical endopytic fungi hyperdiverse? Ecol. Lett. 3: 267–274.
Brase S., Encinas A., Keck J., Nising C. F. 2009. Chemistry and biology of mycotoxins and related fungal metabolites. Chem. Rev. 109: 3903–3990.
[CLSI] Clinical and Laboratory Standards Institute 2005. Performance standards for antimicrobial susceptibility testing fifteenth informational supplement. Document M100-S15, Vol.25, No.1. Clinical and laboratory standards institute, Wayne, Pa.
Damjan J., Samo K., Maja J., Katja S., Borut S. 2007. Antibacterial activity in higher fungi (Mushrooms) and Endophytic fungi from Slovenia. Pharm. Biol. 45: 700–706.
Demain A. L, Sanchez S. 2009. Microbial drug discovery: 80 years of progress. J. Antibiot. 62: 5–16.
Doyle J. J., Doyle J. L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11–15.
Fridkin S. K., Gaynes R. P. 1999. Antimicrobial resistance in intensive care units. Clin. Chest Med. 20: 303–316.
Fernandes M. R. V., Silva T. A. C., Pfenning L. H., Costa-Neto C. M., Heinrich T. A., Alencar S. M., Lima M. A., Ikegaki M. 2009. Biological activities of the fermentation extract of the endophytic fungus Alternaria alternata isolated from Coffea arabica L. Braz. J. Pharm. Sci. 45: 677–685.
Gu W. 2009. Bioactive metabolites from Alternaria brassicicola ML-P08, an endophytic fungus residing in Malus halliana. World J. Microb. Biotech. 25: 1677–1683.
Kjer J., Wray V., Edrada-Ebel R., Ebel R., Pretsch A., Lin W., Proksch P. 2009. Xanalteric acids I and II and related phenolic compounds from an endophytic Alternaria sp. isolated from the mangrove plant Sonneratia alba. J. Nat. Prod. 72: 2053–2057.
Liu Z., Zhu J., Sun B., Liu S., Geng S., Liu X., Li C. L. 2007. Alternol inhibits proliferation and induces apoptosis in mouse lymphocyte leukemia (L1210) cells. Mol. Cell Biochem. 306: 115–122.
Lowy F. D. 1998. Staphylococcus aureus infections. New Engl. J. Med. 339: 520–532.
Mammina C., Cala C., Bonura C., Carlo P. D., Aleo A., Fasciana T., Giammanco A. 2012. EPI-MRSA Working Group: Polyclonal non multi-resistant Staphylococcus aureus isolates from clinical cases of infection occurring in Palermo, Italy, during a one-year surveillance period. Ann. Clin. Microbiol. Antimicrob. 11: 17.
Nagaveni S., Rajeshwari H., Oli A. K., Patil S. A., Chandrakanth R. K. 2011. Widespread emergence of multidrug resistant Pseudomonas aeruginosa isolated from CSF samples. Indian J. Microbiol. 51: 2–7.
[NNIS] National Nosocomial Infections Surveillance 2002. National Nosocomial Infections Surveillance (NNIS) System report, data summary from January 1992 to June 2002, issued August 2002. Am. J. Infect. Control 30: 458–475.
Ravikumar S., Gnanadesigan M., Thajuddin N., Chakkaravarthi V. S. D., Banerjee B. M. 2010. Anticancer property of sponge associated actinomycetes along Palk Strait. J. Pharm. Res. 3: 2415–2417.
Stroble G., Daisy B., Castillo U., Harper J. 2004. Natural products from endophytic microorganisms. J. Nat. Prod. 67: 257–268.
Tenover F. C. 2006. Antimicrobial Resistance in Bacteria. Am. J. Med. 119: 3–10.
Tsuge T., Harimoto Y., Akimitsu K., Ohtani K., Kodama M., Akagi Y., Egusa M., Yamamoto M., Otani H. 2013. Host-selective toxins produced by the plant pathogenic fungus Alternaria alternata. FEMS Microbiol. Rev. 37: 44–66.
Wang J., Zhou J. Y., Qu T. T., Shen P., Wei Z. Q. 2010. Molecular epidemiology and mechanisms of carbepenem resistance in Pseudomonas aeruginosa isolates from Chinese hospitals. Int. J. Antimicrob. Ag. 35: 486–491.
Wang W., Wang Y., Tao H., Peng X., Liu P., Zhu W. 2009. Cerebrosides of the halotolerant fungus Alternaria raphani isolated from a sea salt field. J. Nat. Prod. 72: 1695–1698.
White R. L., Burgess D. S., Mandruru M., Bosso J. A. 1996. Comparison of three different in vitro methods of detecting synergy: Time-kill, checkerboard and E-test. Antimicrob. Agents Chemother. 40: 1914–1918.
White T. J., Bruns T. D., Lee S., Taylor J. W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: a Guide to Methods and Applications (Innis M. A., Gelfand D., Sninsky J. S., White T. J., Eds.), pp. 315–322, Academic, San Diego.
Wright G. D. 2007. The antibiotic resistome: the nexus of chemical and genetic diversity. Nat. Rev. Microbiol. 5: 175–186.
Ya-Tuan M., Li-Rui Q., Wen-Quan S., An-Ling Z., Jin-Ming G. 2010. Metabolites produced by an endophyte Alternaria alternata isolated from Maytenus hookeri. Chem. Nat. Compd. 46: 3.
Zaferanloo B., Quang T. D., Daumoo S., Ghorbani M. M., Mahon P. J., Palombo E. A. 2014. Optimization of protease production by endophytic fungus, Alternaria alternata isolated from an Australian native plant. World J. Microbiol. Biotechnol. Doi:10.1007/s11274-014-1598-z.
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