Semi-specific intron-exon splice junction markers in bryophyte studies
PDF

Keywords

intron-exon splice junction markers
bryophytes

How to Cite

Sawicki, J., & Szczecińska, M. (2007). Semi-specific intron-exon splice junction markers in bryophyte studies. Biodiversity: Research and Conservation, (5-8), 25–30. https://doi.org/10.14746/biorc.2007.5-8.5

Number of views: 24


Number of downloads: 14

Abstract

We present a protocol for use of intron-exon splice junction (ISJ) markers in studies on bryophytes. The advantages and disadvantages of the method are discussed and compared with other PCR-based markers like AFLP, ISSR and RAPD.

https://doi.org/10.14746/biorc.2007.5-8.5
PDF

References

ASHTON N. W., ANTONISHYN N. A. & BAKER K. E. 1994. Molecular phylogenetic aspects of genetical and physiological studies of moss development. J. Hattori Bot. Lab. 76: 41-57.

BĄCZKIEWICZ A., SAWICKI J., BUCZKOWSKA K., POLOK K. & ZIELIŃSKI R. 2008. Application of different DNA markers in studies on cryptic species of Aneura pinguis (Hepaticae, Metzgeriales). Cryptogamie briologie 29(1): 3-21.

BOISSELIER-DUBAYLE M. C. & BISHLER H. 1994. A combination of molecular and morphological characters for delimitation of taxa in European Porella. J. Bryol. 18: 1-11. DOI: https://doi.org/10.1179/jbr.1994.18.1.1

BOISSELIER-DUBAYLE M. C., JUBILER M. F., LEJEUNE B. & BISCHLER H. 1995. Genetic variability in the three subspecies of Marchantia polymorpha (Hepaticae): izozymes, RFLP and RAPD markers. Taxon 44: 363-376. DOI: https://doi.org/10.2307/1223406

CHMIEL J. & POLOK K. 2005. Use of DNA markers for assessing the genetic identity between native and introduced stone pine (Pinus cembra) in the Tatra Mountains. In: W. PRUS-GŁOWACKI W. (ed.). Variability and Evolution - New Perspectives, seria Biologia 72: 137-147. UAM Poznań.

CRONBERG N. 1995. Isozyme electrophoresis of Sphagnum: an analysis and methodology. Lindbergia 20: 40-48.

CRONBERG N. 1996. Isozyme evidence of relationships within Sphagnum sect. Acutifolia (Sphagnaceae, Bryophyta). Pl. Syst. Evol. 203: 41-64. DOI: https://doi.org/10.1007/BF00985236

DOYLE J. J & DOYLE J. L. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13-15. DOI: https://doi.org/10.2307/2419362

GUNNARSSON U., HASSEL K. & SODERSTROM L. 2005. Genetic structure of endangered peat moss Sphagnum angermanicum in Sweden: a results of historic and contemporary processes? Bryologist 108: 194-203. DOI: https://doi.org/10.1639/0007-2745(2005)108[0194:GSOTEP]2.0.CO;2

HASSEL K. & GUNNARSSON U. 2003. The use of inter simple sequence repeats (ISSR) in bryophyte population studies. Lindbergia 28: 152-157.

KRZAKOWA M. & SZWEYKOWSKI J. 1977. Peroxidases as taxonomic markers in two critical Pellia taxa (Hepaticae, Pelliaceae). Bull. Acad. Polon. Sci., Ser. Sci. Biol. 25: 203-204.

LEWANDOWSKA K. 2001. Estimation genetic similarity of Lolium perenne L. and L. multiflorum Lam. on the basis of molecular markers. Ph. D. Thesis, University of Warmia and Mazury in Olsztyn.

ODRZYKOSKI I. J. & SZWEYKOWSKI J. 1991. Genetic differentiation without concordant morphological difference in the thallose liverwort Conocephalum conicum. Pl. Syst. Evol. 178: 135-151. DOI: https://doi.org/10.1007/BF00937961

PEDERSEN N., RUSSELL S. J. & NEWTON A. E. 2006. A novel molecular protocol for rapid extraction of DNA from bryophytes and the utility of direct amplification of DNA from a single dwarf male. Bryologist 109(2): 257-264. DOI: https://doi.org/10.1639/0007-2745(2006)109[257:ANMPFT]2.0.CO;2

PEREZ T. J., ALBORNOZ J. & DOMINGUEZ A. 1998. An evaluation of RAPD fragment reproducibility and nature. Mol. Ecol. 7: 1347-1357. DOI: https://doi.org/10.1046/j.1365-294x.1998.00484.x

POLOK K. 2005. Evolutionary status of close related Lolium L. taxa. In: W. PRUS-GŁOWACKI (ed.). Variability and Evolution n New Perspectives, seria Biologia 72: 195-207. UAM Poznań.

POLOK K., HOŁDYŃSKI CZ., SAWICKI J., SZCZECIŃSKA M. & ZIELIŃSKI R. 2005a. Genetic similarity of Polish Sphagnum species on the base of RAPD and ISJ makers. In: W. PRUS-GŁOWACKI W. (ed.). Variability and Evolution - New Perspectives, seria Biologia 72: 209-216. UAM Poznań.

POLOK K., HOŁDYŃSKI CZ., SZCZECIŃSKA M., SAWICKI J. & ZIELIŃSKI R. 2005b. Genetic similarity of Polish Polygonatum species on the base of RAPD and ISJ markers. In: W. PRUS-GŁOWACKI (ed.). Variability and Evolution - New Perspectives, seria Biologia 72: 217-223. UAM Poznań.

POLOK K., SAWICKI J., KUBIAK K., SZCZECIŃSKA M., KORZEKWA K., SZANDAR K. & ZIELIŃSKI R. 2005c. Genetic divergence within Pellia endiviifolia (Dicks.) Dum. from Poland. In: W. PRUS-GŁOWACKI W. (ed.). Variability and Evolution - New Perspectives, seria Biologia 72: 241-252. UAM Poznań.

SAWICKI J. 2006. Assesment of taxonomical position of selected Sphagnum species (section Acutifolia) on the base of molecular markers. Ph. D. Thesis, University of Warmia and Mazury in Olsztyn.

SAWICKI J., NADOLSKI Ł. & ZIELIŃSKI R. 2005. Identification of Conocephalum conicum and C. salebrosum on the basis of DNA markers. In: B. JACKOWIAK & Z. CELKA (eds.). Taksonomia, chorologia i ekologia roślin w dobie zagrożenia różnorodności biologicznej. Materiały konferencji naukowej dedykowanej Profesorowi dr hab. Waldemarowi Żukowskiemu z okazji 70-lecia urodzin, p. 197. UAM Poznań.

SCOTT K. M. & CRANDALL-STOTLER B. 2002. RAPD polymorphism as an indicator of population structure, breeding system and speciation in Fossombronia. Bryologist 105(2): 225-232. DOI: https://doi.org/10.1639/0007-2745(2002)105[0225:RPAAIO]2.0.CO;2

SNALL T., FOGELQVIST J. & RIBERIO P. J. 2004. Spartial genetic structure in two congeneric epiphytes with different dispersal strategies analysed with three different method. Mol. Ecol. 13: 2109-2119. DOI: https://doi.org/10.1111/j.1365-294X.2004.02217.x

STENOIEN H. K. 1999. Protocols for DNA isolation and random amplified polymorphic DNA (RAPD) analysis on Sphagnum. Lindbergia 24: 43-37.

STENOIEN H. K. & FLATBERG K. I. 2000. Genetic variability in the rare Norwegian peat moss Sphagnum troendelagicum. Bryologist 103: 794-801. DOI: https://doi.org/10.1639/0007-2745(2000)103[0794:GVITRN]2.0.CO;2

STENOIEN H. K. & SASTAD S. M. 1999. Genetic structure in three haploid peat mosses (Sphagnum). Heredity 82: 391-400. DOI: https://doi.org/10.1038/sj.hdy.6884940

STUMMER B. E., ZANKER T. & SCOTT E. S. 2000. Genetic diversity in population Uncinula necator: comparison of RFLP- and PCR-based approaches. Mycological Research 104: 44-52. DOI: https://doi.org/10.1017/S0953756299001070

SZCZECIŃSKA M. 2006. Genetic diversity of lowland and montane populations of Polygonatum verticillatum (L.). All. Ph. D. Thesis, University of Warmia and Mazury in Olsztyn.

SZCZECIŃSKA M., SAWICKI J., POLOK K., HOŁDYŃSKI CZ. & ZIELIŃSKI R. 2006. Comparison of three Polygonatum species from Poland based on DNA markers. Ann. Bot. Fenn. 43(5): 379-388.

VANDERPORTEN A. & TIGNON M. 2000. Amplified fragments length polymorphism between populations Amblystegium tenax exposed to contrasting water chemistries. J. Bryol. 22: 257-262. DOI: https://doi.org/10.1179/jbr.2000.22.4.257

VEKEMANS X. & JACQUEMART A. L. 1997. Perspectives on the use of molecular markers in plant population biology. Belgian J. Bot. 129: 91-100.

WEINING S. & LANGRIDGE P. 1991. Identification and mapping of polymorphisms in cereals based on polymerase chain reaction. Theor. Appl. Genet. 82: 209-216. DOI: https://doi.org/10.1007/BF00226215

WEINING S. & HENRY R. J. 1995. Molecular analysis of the DNA polymorphism of wild barley (Hordeum spontaneum) germplam using the polymerase chain reaction. Genetic Resource and Crop Evolution 42(3): 273-280. DOI: https://doi.org/10.1007/BF02431262

WERNER O., ROS R. M. & GUERRA J. 2002. Direct amplification and NaOH extraction: two rapid and simple methods for preparing bryophyte DNA for polymerasae chain reaction (PCR). J. Bryol. 24: 127-131. DOI: https://doi.org/10.1179/037366802125000980

WYATT R., ODRZYKOSKI I. J. & STONEBURNER A. 1989. Bryophyte isozymes: systematic and evolutionary implications. In: D. E. SOLTIS & P. S. SOLTIS (eds.). Isozymes in plant biology, pp. 221-240. Dioscorides Press. DOI: https://doi.org/10.1007/978-94-009-1840-5_12

ZIELIŃSKI R. 1987. Genetic variation of the liverwort genus Pellia with special reference to central European territory. Rozpr. Stud. Uniw. Szczec. 108(24): 1-297.

ZIELIŃSKI R. & POLOK K. 2005. Molecular evolution and plant taxonomy. In: W. PRUS-GŁOWACKI (ed.). Variability and Evolution - New Perspectives, seria Biologia 72: 37-55. UAM Poznań.