Variability of Anthoxanthum species in Poland in relation to geographical-historical and environmental conditions: isozyme variation
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Keywords

Poaceae
Anthoxanthum alpinum
Anthoxanthum aristatum
Anthoxanthum odoratum
Poland
isozymes
genetic differentiation

How to Cite

Drapikowska, M. (2013). Variability of Anthoxanthum species in Poland in relation to geographical-historical and environmental conditions: isozyme variation. Biodiversity: Research and Conservation, 30, 63–93. https://doi.org/10.2478/biorc-2013-0011

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Abstract

Variation of 9 isozyme systems was studied in Polish populations of 3 species of the genus Anthoxanthum: the native A. odoratum s. str. L. and A. alpinum Á. Löve & D. Löve, as well as the alien A. aristatum Boiss. Results of this study show that A. odoratum is characterized by a high isozyme variability of lowland populations, weakly correlated with habitat type, and partial genetic distinctness of montane populations. Moreover, 5 isozyme markers have been identified (Pgi-2, Dia-2, Mdh, Idh, Pgm) for the allopolyploid A. odoratum. Populations of A. aristatum are highly polymorphic (P = 98%). The observed isozyme differentiation of its populations (FST = 0.087) is low and gene flow between them (Nm = 5.314) is high. The genetic variation reflects environmental variation only to a small extent and is not significantly related to the phase of chorological expansion of this species. Altitudinal vicariants, A. alpinum and A. odoratum, are characterized by morphological and isozymatic distinctness, indicating their reproductive isolation. In populations of A. alpinum, polymorphism is high (P = 76.92%), differentiation among populations is moderate (FST = 0.198), and gene flow between populations along the altitudinal transect (Nm = 1.709) is relatively low

https://doi.org/10.2478/biorc-2013-0011
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References

Abha J., Roy A. K., Kaushal P., Malaviya D. R. & Zadoo S. N. 2006. Isozyme banding pattern and estimation of genetic diversityamong Guinea grass germplasm. Genetic Resources and Crop Evolution 53: 339-347.

Angelov G. B. & Ivanova T. A. 2012. Isoenzyme variation and genetic affinities among four species of the genus Festuca L. (Poaceae). Biodiv. Res. Conserv. 28: 3-8. Antonovics J. 1972. Population dynamics of Anthoxanthum odoratum on a zinc mine. Journal of Ecology 60: 351-365.

Badr A., Sayed-Ahmed H., El-Shanshou ri A. & Watson L. E. 2002. Ancesors of white clower (Trifolium repens L.) as revealed by isozyme polymorphism. Theor. Appl. Genet. 106: 143-148.

Barrett S. C. H. & Shore J. S. 1989. Isozyme variation in colonizing plants. In: D. Soltis & P. Soltis (eds.). Isozymes in Plant Biology, pp. 106-126. Dioscorides Press, Portland, Oregon.

Borrill M. 1963. Experimental studies of evolution in Anthoxanthum (Graminae). Genetica 34(1): 183-210.

Blossey B. & R. Nötzold. 1995. Evolution of increased competitive ability in invasive nonindigenous plants: a hypothesis. Journal of Ecology 83: 887-889.

Bretagnolle F. 2001. Pollen production and spontaneous polyploidization in diploid populations of Anthoxanthumalpinum. Biol. J. Linn. Soc. 72: 241-247.

Cohen J. 1988. Statistical Power Analysis for the Behavioral Sciences. 596 pp. Lawrence Erlbaum Associates.

Dixon J. M. 2002. Biological flora of the British Isles: Brizamedia L. J. Ecol. 90: 737-752.

Djebaili S. 1990. Syntaxonomie des groupements prèforetsiers et steppiques de l’Algérie aride. Ecologia Mediterranea 16: 231-244.

Drapikow ska M. 2013. Variability of Anthoxanthum species in Poland in relation to geographical-historical and environmental conditions: morphological and anatomical variation. Biodiv. Res. Conserv. 30: 3-61.

Drapikowska M. & Krzakowa M. 2009. Morphological an biochemical variation among common reed (Phragmitesaustralis) populations in northwest Poland. Oceanological and Hydrobiological Studies 38(2): 29-38.

Drapikowska M., Celka Z., Szkudlarz P. & Jackowiak B. 2011. Zmienność morfologiczna populacji Anthoxanthumodoratum (Poaceae) na siedliskach o zróżnicowanymstopniu antropogenicznego przekształcenia. Fragm. Flor. Geobot. Polonica 18(2): 281-293.

Drapikowska M., Leśniewska K., Hasterok R., Szkudlarz P., Celka Z. & Jackow iak B. 2013. Variability of stomata and 45S and 5S rDNAs loci characteristics in two species of Anthoxanthum genus: A. aristatum and A. odoratum (Poaceae). 2013. Acta Biologica Hungarica 64(3).

Ellmer M., Predice H. C. & Anderson S. 2011. The structuring of quantitative genetic variation in a fragmented population of Briza media (Poaceae). Evol. Ecol. 25: 509-523.

Enzyme [Internet]. Enzyme nomenclature database. 2010. [cited 2010 Aug 8]; Available from: http://www.expasy.ch/enzyme/

Ellstrand N. C. & Schierenbeck K. 2000. Hybridization as a stimulus for the evolution of invasiveness in plants? Proc Nat Acad Sci USA 97: 7043-7050.

Excoffier L., Smuse P. E. & Quatro J. M. 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrian DNA restriction data. Genetics 131: 479-491.

Felber F. 1988. Distribution des cytodemes d’Anthoxanthumodoratum L. s. lat. en France et dans les regions limitrophes. Bulletin de Societe Botanique Francaise. 135. Lettres Botanique 131: 281-293.

Felber-Girard M., Felber F. & Buttler A. 1996. Habitat differentiation in narrow hybrid zone between diploid and tetraploid Anthoxanthum alpinum. New Phytol. 133: 531-540.

Fernández-Moya J., San Miguel-Ayanz A., Cañellas I. & Gea-Izquierdo G. 2010. Variability in Mediterranean annual grassland diversity driven by small-scale changes in fertility and radiation. Plant Ecol 212(5): 865-877. DOI 10.1007/s11258-010-9869-8.

Filipová L. & Krahulec F. 2006. The transition zone between Anthoxanthum alpinum and A. odoratum in the Karkonose Mts. Preslia 78: 317-330.

Flegrova M. & Krahulec F. 1999. Anthoxanthum odoratum and A. alpinum: Life history parameters at two different altitudes. Folia Geobotanica 34(1): 19-31.

Freeland J. R., Biss P., Conrad K. F. & Silvertown J. 2010. Selection pressures have coused genome-wide population differentiation of Anthoxanthum odoratum despite the potential for high gene flow. J. Evol. Biol. 23(4): 776-782. DOI 10.1111/j.1420-9101.210.01947.x

Freeland J. R., Biss P. & Silvertown J. 2012. Contrasting patterns of pollen and seed flow influence the spatial genetic structure of sweet vernal grass (Anthoxanthumodoratum) populations. J. Hered. 103(1): 28-35. DOI 10.1093/jhered/esr111. Epub 2011 Oct 13.

Fowler N. 1982. Competition and coexistence in a North Carolina Grassland. III. Mixtures of component species. J. Ecology 70: 77-92.

Gifford A. L. S., Ferdy J. B. & Molofsky J. 2002. Genetic composition and morphological variation among populations of the invasive grass, Phalaris arundinacea. Can. J. Bot. 80: 779-785.

Goodman M. M., Stub er C. W., Lee C. N. & Johnson F. M. 1980. Genetic control of malate dehydrogenase isozymes in maize. Genetics 94: 153-168.

Gottlieb L. D. 1981. Electrophoretic evidence and plant populations. Progress in Phytochemistry 7: 1-46.

Hahn T., Kettle C. J., Jaboury Ghazoul E., Frei R., Matter P. & Pluess A. R. 2012. Patterns of Genetic Variation across Altitude in Three Plant Species of Semi-Dry Grasslands. PLoS ONE 7(8): e41608. DOI 10.1371/ journal.pone.0041608.

Hamrick J. L. & Godt M. J. W. 1989. Allozyme diversity in plant species. In A. H. D. Brown M. T. Clegg A. L. Kahler & Weir B. S. (eds.). Plant population genetics, breeding and genetic resources, pp. 43-63. Sinauer, Sunderland, MA.

Hamrick J. L. & Loveless M. D. 1986. Allozyme variation in tropical trees: Techniques and preliminary results. Biotropica 18: 201-207.

Hamrick J. L. & Godt M. J. W. 1996. Effects of life history traits on genetic diversity in plant species. Phil. Trans. Roy. Soc. London Biol. Sci. 351: 1291-1298.

Jones K. 1964. Chromosomes and the nature and origin of Anthoxanthumodoratum L. Chromosoma 15: 248-274.

Kapeluszny J. & Haliniarz M. 2010. Ekspansywne i zagrożone gatunki flory segetalnej w środkowo-wschodniej Polsce. Annales Universitas Mariae-Curie Skłodowska Lublin Polonia. 65(1): 26-33. DOI 10.2478/ v10081-010-0004-2

Kimura M. & Crow J. F. 1964. The number of alleles that can be maintained in a finite population. Genetics 49: 725-38.

Krzakowa M. 1996. Genetic diversity of Phragmites australis (Cav.) Trin. ex Steud. Revealed by electrophoretically detected differences in peroxidases. In: C. Obinger, U. Burner, R. Eberman, C. Penel & H. Greppin (eds.). Plant peroxidases: biochemistry and physiology, pp. 184-189. University of Geneva.

Krzakowa M. & Celka Z. 2007. Intrapopulation variation of Calamagrostis arundinacea L. (Roth.) revealed by electrophoretically detected ten izoenzyme systems. Biodiv. Res. Conserv. 5-8: 5-9.

Krzakowa M. & Celka Z. 2008. Intraspecific differentiation of Reed Grass Calamagrostis arundinacea (Poaceae) populations revealed by peroxidase allozymes. Acta Soc. Bot. Pol. 77(4): 299-304.

Krzakowa M. & Dunajski A. 2007. Genetic differences and hybridization between Calamagrostis arundinacea and C. villosa (Poaceae) in the anemo-orographic (AO) system in the Karkonosze Mountains. Biochemical Systematics and Ecology 35: 23-28.

Kubešová M., Moravc ová L., Duda J., Jarošik W. & Pyšek P. 2010. Naturalized plants have smaller genomes than their non-invading relatives: a flow cytometric analysis of the Czech alien flora. Preslia 82: 81-96.

Lack A. J. & Kay Q. O. N. 1986. Phosphoglucose isomerase (EC 5.3.1.9) isozymes in diploid and tetraploid Polygala species: evidence for gene duplication and diversification. Heredity 56: 111-118.

Lange M. 1995. Spearman`s Principle. Brit. J. Phil. Sci. 46: 503-521.

Latowski K. 2005. Ecological-biological reasons and sources of the invasive propensity of Anthoxanthum aristatum Boiss. Thaiszia - J. Bot. Košice, 15, Suppl. 1: 143-152.

Lavergne S. & Molosky J. 2007. Increased genetic variation and evolutionary potential drive the success of invasive grass. PNAS 104(10): 3883-3888.

Lumaret R. 1984. The role of ploidy in the adaptive significance of polymorphism at the GOT locus in the Dactilis glomerata L. complex. Heredity 52: 153-169.

Manly B. F. J. 1985. The Statistics of Natural Selection on Animal Populations. 500 pp. Chapman and Hall, London.

Mirek Z. & Piękoś-Mirkowa H. 2003. Grasses of mountains of Poland. In: L. Frey (ed.). Problems of grass biology, pp. 95-118. W. Szafer Institute of Botany, Polish Academy of Sciences, Kraków.

Mirek Z., Piękoś-Mirkowa H., Zając A. & Zając M. 2002. Flowering plants and pteridophytes of Poland. A checklist. In: Z. Mirek (ed.). Biodiversity of Poland, 1, 442 pp. W. Szafer Institute of Botany, Polish Academy of Sciences, Kraków.

Mitton J. B. 1989. Selection in Natural Populations. Oxford University Press, Oxford-New York.

Mizianty M. 1991. Biosystematic studies on Dactylis (Poaceae). 2. Original research. 2.2 Cytological differentiation of the genus in Poland. Fragm. Flor. Geobot. 36: 301-320.

Nei M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89(3): 583-590.

Nyberg Berglund A. B., Saura A. & Westerbergh A. 2006. Electrophoretic Evidence For Disomic Inheritance And Allopolyploid Origin Of the Octoploid Cerastiumalpinum (Caryophyllaceae). Journal of Heredity 97(3): 296-302. DOI 10.1093/jhered/esj029.

Oja T. & Jaa ska V. 1998. Allozyme diversity and phylogenic relationships among diploid annual bromus (Bromus Poaceae). Ann. Bot. Fennici 35: 123-130.

Ohsawa T. & Ide Y. 2008. Global patterns of genetic variation in plant species along vertical and horizontal gradients on mountains. Global Ecology and Biogeography 17: 152-163.

Peakall R. & Smou se P. E. 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes. 6(1): 288-295. http://dx.doi.org/10.1111/j.1471-8286.2005.01155.x

Pimentel M., Catalan P. & Sahuquillo E. 2010. Morphological and molecular taxonomy of the annual diploids Anthoxanthum aristatum and A. ovatum (Poaceae) in the Iberian Peninsula. Evidence of introgression in natural populations. J. Linn. Soc. Bot. 164: 53-71.

Pimentel Pereira M., Estevez Perez G. & Sahuquillo Balbuena E. 2007. European Sweet Vernal Grasses (Anthoxanthum: Poaceae, Pooideae, Aveneae): A Morphometric Taxonomical Approach. Systematic Botany 32(1): 43-59.

Pimentel M. & Sahuquillo E. 2008. Relationships between the close congeners Anthoxanthum odoratum and A. alpinum (Poaceae, Pooideae) assessed by morphological and molecular methods. J. Linn. Soc. Bot. 156: 237-252.

Prentis P. J., Wilson J. R. U., Dormontt E. E., Richardson D. M. & Lowe A. J. 2008. Adaptative evolution in invasive species. Trends in Plant Sciences 13(6): 288-294.

Reeves G., Francis D., Davies M. S., Rogers H. J. & Hodkinson T. 1998. Genome size is negatively correlated with altitude in natural populations of Dactylis glomerata. Annals of Botany 82, Suppl. A: 99-105.

Rozmus M. 1958. Cytological investigations of Anthoxanthumalpinum L. et L. a new species of the flora in Poland. Acta Biol. Cracov. ser. Bot. 1: 171-184.

Silvertown J., Poulton. P., Johnson E., Grand E., Heard M. & Biss P. M. 2006. The parkgrassexperiment 1856-2006: its contribution to ecology. Journal of Ecology 94:801-814.

Shahi B. B., Chu Y. E. & Oka H. J. 1969. Analysis of genes controlling peroxidase izozymes in Oryza sativa and O. perennis. Can. J. Genet. 15: 845-853.

Skrajna T. & Skrzypc zyńska J. 2007. Wybrane cechy biologiczne i występowanie A. aristatum Boiss. Na WysoczyźnieKałuszyńskiej. Annales Univeritatis Mariae Curie-Skłodowska Lublin-Polonia 62(2):145-155.

Sneath P. H. A. & Sokal R. R. 1973. Numerical taxonomy. 359 pp. Freeman W. H., San Francisco.

Stoöcklin J. K. P. & Pluess A. 2009. Genetic diversity, phenotypic variation and local adaptation in the alpine landscape: case studies with alpine plant species. Bot. Helv. 119: 125-133.

Szmeja K. 1996. Anthohanthum aristatum Boiss. - Ekspansywny chwast pól uprawnych Równiny Charzykowskiej. Zesz. Nauk. ATR Bydgoszcz 196, Rol. 38: 193-196.

Szweykow ski J. & Odrzyko ski I. 1990. Chemical differentiation of Aneura pinguis (L.) Dum. (Hepaticae, Aneuraceae) in Poland and some comments on application of enzymatic markers in bryology. Bryophytes. Their chemistry and taxonomy 437-448.

Śliwińska E. 2008. Zastosowanie cytometrii przepływowej do oznaczania DNA u roślin. Postępy Biologii Komórki 35(24): 165-176.

Tokarska-Guzik B. 2005. The Establishment and Spread of Alien Plant Species (Kenophytes) in the Flora of Poland. Prace naukowe Uniw. Śląskiego w Katowicach 2372: 1-192.

Weeden N. F. & Gottlieb L. D. 1980. The genetics of chloroplast enzymes. J. Heredity. 71: 392-396.

Wendel J. F. & Weeden N. F. 1989. Visualization and interpretation of plant isozymes. In: D. E. Soltis & P. S. Soltis (eds.). Isozymes in Plant Biology, pp. 5-45. Dioscorides Press, Portland, Oregon.

Wright S. 1951. The genetic structure of populations. Annals of Eugenics 15: 323-354.

Yeh C. F. & Boule T. 2000. Popgene 1.32: A free program for the analysis of genetic variation among and within populations using co-dominant and dominant markers. Department of Renewable Resources at University of Alberta, Canada.

Zhao Gui F., Felber F. & Kuepfer P. 2001. Subpopulation differentiation of Anthoxanthum alpinum (Poaceae) along an altitudinal gradient detected by random amplifiedpolymor phic DNA. Acta Phytotaxonomica Sinica 38: 64-70.

Zeroua l-Humbert-Droz C. & Felber F. 1999. Evidence from isozyme analysis of autopolyploidy in Anthoxanthumalpinum A. & D. Love. Botanica Helvetica 109: 217-227.

Zeidler M. 2000. Electrophoretic analysis of plant isozymes. Acta Universitas Palackianae Olomucensis Facultas Rerum Naturalium Biologica 38: 7-16.