Keywords
liverworts
complex species
Calypogeia
PCR-RFLP
SCAR
How to Cite
Number of views: 20
Number of downloads: 12
Abstract
Currently, two subspecies are formally recognized within Calypogeia fissa: C. fissa subsp. fissa occurring in Europe and C. fissa subsp. neogea known from North America. Genetic studies have revealed a complex structure of this species. Within the European part of distribution, three genetically distinct groups PS, PB and G are distinguished. The combination of the SCAR marker Cal04 and PCR-RFLP markers with three restriction enzymes (SmaI, TaqI and TspGWI) allowed the recognition of all groups within the C. fissa complex. The TaqI enzyme recognizing the restriction sites in the PCR product of SCAR marker Ca104 turned out to be the best marker
References
Avise J. C. 2004. Molecular Markers, Natural History, and Evolution. Second Edition. 684 pp. Sinauer Association, Inc.
Bell D., Long D. G., Forrest A. D., Hollingsworth M. L., Blom H. H. & Hollingsworth P. M. 2012. DNA barcoding of European Herbertus (Marchatiopsida, Herbertaceae) and the discovery and description of a new species. Mol. Ecol. Res. 12: 36-47.
Bischler H. 1963. The genus Calypogeia Raddi in Central and South America. I-III. Candollea 18: 19-128.
Bischler H. & Boisselier-Dubayle M. C. 2000. New approaches to the systematics of liverworts. Nova Hedwigia 70: 37-44.
Boisselier-Duba yle M. C., Jubier M. F., Lejeune B. & Bisch - ler H. 1995. Genetic variability in the three subspecies of Marchantia polymorpha (Hepaticae): isozymes RFLP and RAPD markers. Taxon 44: 363-376.
Buczkowska K. 2004. Genetic differentiation of Calypogeia fissa (L.) Raddi (Hepaticae, Jungermanniales) in Poland. Pl. Syst. Evol. 247: 187-201.
Buczkowska K. & Bączkiewicz A. 2011. New taxon of the genus Calypogeia (Jungermanniales, Hepaticae) in Poland. Acta Soc. Bot. Pol. 80: 327-333.
Buczkowska K. & Dab ert M. 2011. The development of species-specific SCAR markers for delimitation of Calypogeia species. J. Bryol. 33: 291-299.
Buczkowska K., Gonera P. & Hornik B. 2012a. PCR-based molecular markers for identyfication of taxa from the Calypogeia fissa complex (Jungermanniopsida, Calypogeiaceae). Biodiv. Res. Conserv. 28: 9-18.
Buczkowska K., Sawicki J., Szczecińska M., Klama H. & Bączkiewicz A. 2012b. Allopoliploid speciation of Calypogeia sphagnicola (Jungermanniopsiada, Calypogeiaceae) based on isozyme and DNA markers. Pl. Syst. Evol. 298: 549-560.
Buczkowska K., Sawicki J., Szczecińska M., Rosadziński S., Rabska M. & Bączkiewicz A. 2011. Two morphologically distinct groups of the Calypogeia fissa complex were found in Europe. Biodiv. Res. Conserv. 23: 7-19.
Chen S., Yao H., Han J., Liu C., Song J., Shi L., Zhu Y., Ma X., Gao T., Pang X., Luo K., Li Y., Li X., Jia X., Lin Y. & Leon C. 2010. Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS One 5:e8613.
Damsholt K. 2002. Illustrated Flora of Nordic Liverworts and Hornworts. 837 pp. Nordic Bryological Society, Lund.
Feldberg K., Groth H., Wilson R., Schäfer-Verwimp A. & Heinrichs J. 2004. Cryptic speciation in Herbertus (Herbertaceae, Jungermanniopsida): Range and morphology of Herbertus sendtneri inferred from nrITS sequences. Plant Syst. Evol. 249: 247-261.
Fuselier L., Davison P. G., Clements M., Sha w B., Devos N., Heinrich s J., Hentsch el J., Sab ovljevic M., Szovenyi P., Sch uette S., Hofba uer W & Sha w A. J. 2009. Phylogeographic analysis reveal distinct lineages of the liverworts Metzgeria furcata (L.) Dumort. and Metzgeria conjugata Lindb. (Metzgeriaceae) in Europe and North America. Biol. J. Linn. Soc. 98: 745-756.
Grolle R. & Long D. G. 2000. An annotated check-list of the Hepaticae and Anthocerotae of Europe and Macaronesia. J. Bryol. 22: 103-140.
Hentschel J., Zhu R.-L., Long D. G., Davison P. G., Schneider H., Gradstein S. R. & Heinrichs J. 2007. A phylogeny of Porella (Porellaceae, Jungermanniopsida) based on nuclear and chloroplast DNA sequences. Molec. Phylog. Evol. 45:693-705.
Heinrichs J., Klugmann F., Hentschel J. & Schneider H. 2009. DNA taxonomy cryptic speciation and diversification of the Neotropical-African liverwort Marchsinia brachiata (Lejeuneaceae Porellales). Molec. Phylog. Evol. 53: 113-121.
Heinrichs J., Hentschel J., Bombosch A., Fiebig A., Reise J., Edelma nn M., Kreier H. P., Sch äfer-Verwimp A., Caspa ri S., Schmidt A. R., Zhu R. L., Konrat M., Shaw B. & Shaw A. J. 2010. One species or at least eight? Delimitation and distribution of Frullania tamarisci (L) Dumort s. l. (Jungermanniopsida Porellales) inferred from nuclear and chloroplast DNA markers. Molec. Phylog. Evol. 56: 1105-1114.
Inoue H. 1975. Two new species of Calypogeia Raddi from Japan. Bull. Natl. Sci. Mus. Ser. B 1:135-140.
Jankowiak K., Buczkowska K. & Szweykowska-Kulińska Z. 2005. Successful extraction of DNA from 100-yearold herbarium specimens of the liverwort Bazzania trilobata. Taxon 54: 335-336.
Jankowiak K. & Szweykowska-Kulińska Z. 2004. Organellar inheritance in the allopolyploid liverwort species Porella baueri (Porellaceae): reconstruction historical events using DNA analysis data. Monographs in Molecular Systematic Botany from Missouri Botanical Garden 98: 404-414.
Kreier H. P., Feldberg K., Mahr F., Bombosch A., Schmidt A. R., Zhu R. L., Konrat M., Shaw B., Shaw A. J. & Heinrichs J. 2010. Phylogeny of the leafy liverwort Ptilidium: Cryptic speciation and shared haplotypes between Northern and Southern Hemispheres. Mol. Phylog. Evol. 57: 1260-1267.
Lin T. C. Yeh M. S. Cheng Y. M., Chang L., Lin L. C. & Sung J. M. 2012. Using ITS2 PCR-RFLP to generate molecular markers for authentication of Sophora flavescens Ait. J. Sci. Food Agric. 92: 892-898.
Murray M. & Thomp son W.F. 1980. Rapid isolation of molecular weight plant DNA. Nucleic Acids Res. 8: 4321-4325.
Müller K. 1951-1958. Die Lebermoose Europas In: Dr L. Rabenhorst’s Kryptogamen Flora von Deutschland Österreich und der Schweiz. 3rd ed., pp. 1173-1176. Akademische Verlagsgesellaschaft Geest & Portig K.-G., Leipzig.
Pacak A. & Szweykowska-Kulińska Z. 2003. Organellar inheritance in liverworts. An example of Pellia borealis. J. Mol. Evol. 56: 11-17.
Paton J. 1999. The Livervort Flora of the British Isles. 626 pp. Harley Books, Colchester.
Sawicki J., Plášek V. & Szczecińska M. 2012. Molecular data do not support the current division of Orthotrichum (Bryophyta) species with immersed stomata. J. Syst. Evol. 50: 12-24 doi: 10.1111/j.1759-6831.2011.00168.x
Schumacker R. & Vaňa J. 2005. Identification keys to the liverworts and hornworts of Europe and Macronesia (Distribution and status). 209 pp. Sorus, Poznań.
Schuster R. M. 1969. The Hepaticae and Anthocerotae of North America east of the hundredth meridian vol 2, 1062 pp. Columbia University Press, New York-London.
Szweykowska-Kulińska Z., Pacak A. & Jankowiak K. 2002. New DNA markers for discrimination between closely-related species and for the reconstruction of historical events; an example using liverworts. Cell. Mol. Biol. Lett. 7: 403-416.
Szweykowski J. 2006. An annotated checklist of Polish liverworts. In: Z. Mirek (ed.) Biodiversity of Poland, 4, 114 pp. W. Szafer Institute of Botany, Polish Academy of Sciences, Kraków.
Szweykowski J., Zieliński R., Odrzykoski I. & Buczkowska K. 1995. Geographic distribution of Pellia ssp. (Hepaticaea, Metzgeriales) in Poland based on electrophoretic identification. Acta Soc. Bot. Pol. 64: 59-70.
Szweykowski J., Buczkowska K. & Odrzykoski I. J. 2005. Conocephalum salebrosum (Marchantiopsida, Conocephalaceae) a new Holarctic liverwort species. Plant Syst. Evol. 253: 133-158.
Tamura K., Peterson D., Peterson N., Stecher G., Nei M. & Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol. Biol. Evol. 28: 2731-2739.
Vanderpoorten A., Hedenäs L. & Jac quema rt A.L. 2003. Differentiation in DNA fingerprinting and morphology among species of the pleurocarpous moss genus Rhytidiadelphus (Hylocomniaceae). Taxon 52: 229-236.
Wachowiak W., Bączkiewicz A., Chudzińska E. & Buczkowska K. 2007. Cryptic speciation in liverworts - a case study in the Aneura pinguis complex. Bot. J. Linn. Soc. 155: 273-282.
Yuan C.-I., Lin L.-C., Kuo C.-L. & Chiang M.-Y. 2007. PCRRFLP marker of ribosomal DNA used in detection of adulteration species of Taraxacum mongolicum. Crop Environ. Bioinform 4:285-296.