Keywords
seed sculpture
seed coat
taxonomic analysis
scanning electron microscope
How to Cite
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Abstract
Eight Hypericum species are native to Poland: H. elegans Stephan ex Willd., H. hirsutum L., H. humifusum L., H. maculatum Crantz, H. montanum L., H. perforatum L., H. pulchrum L., and H. tetrapterum Fr. Only seeds of H. elegans were investigated in detail in Poland before, so here we present results of qualitative and quantitative analyses of seed morphology of the other 7 species, based on characters like seed length, width, and shape, seed coat sculpture, shape of epidermal cells of the testa, and number of epidermal cells along the seed axis. The results show that seeds of the studied species are small, 0.56-1.15 mm long and 0.26-0.49 mm wide. In SEM images, seed coat sculpture is reticulate in 5 species, papillate in H. hirsutum, and cup-shaped in H. pulchrum. The differences are caused by the varied final development of the testa epidermis, which constitutes the outer layer of the seed coat. The mean number of epidermal cells along the seed axis ranges from 22 to 33. Results of cluster analysis, based on the agglomeration method and including also published data on seeds of H. elegans, show that the variation in the investigated characters of seeds is reflected in the taxonomic division of the genus into sections.
References
Alonso M. A., Agulló J. C., Villar J. L., Juan A. & Crespo M. B. 2013. Taxonomic relationships in the Hypericum ericoides aggregate (H. sect. Coridium, Hypericaceae). Ann. Bot. Fennici 50: 195-207
Bojňanský V. & Fargašová A. 2007. Atlas of seeds and fruits of Central and East European Flora. The Carpatian Mountains Region. 1025 pp. Springer, Dordrecht.
Crockett S. & Robson N. 2011. Taxonomy and Chemotaxonomy of the Genus Hypericum. Medicinal and Aromatic Plant Science and Biotechnology 5 (Special Issue 1): 1-13.
Hagema n I. 1987. On subspecific taxa of Hypericum empetrifolium (Hypericaceae) from Crete. Pl. Syst. Evol. 155: 165-187.
Kaźmierczakowa R., Bloch-Orłowska J., Celka Z., Cwener A., Dajdok Z., Michalska-Hejeduk D., Pawlikowski P., Szczęśniak E. & Ziarnek K. 2016. Polish red list of pteridophytes and flowering plants. 44 pp. Instytut Ochrony Przyrody PAN, Kraków.
Mártonfi P. 2008. Hypericum dubium Leers - New Data on Taxonomy and Biology. Folia Geobot. 43: 69-82.
Meseguer A. S. & Sanmartín I. 2012. Paleobiology of the genus Hypericum (Hypericaceae): a survey of the fossil record and its palaeogeographic implications Anales del Jardín Botánico de Madrid 69(1): 97-106
Nürk N. M. 2011. Phylogenetic analyses in St. John’s wort (Hypericum). Inferring character evolution and historical biogeography. Ph. D. Thesis, Freien Universität Berlin.
Nürk N. M. & Blattner F. R. 2010. Cladistic analysis of morphological characters in Hypericum (Hypericaceae). Taxon 59: 1495-1507.
Robson N. K. B. 1977. Studies in the genus Hypericum L. (Guttiferae). 1. Infrageneric classification. Bulletin of the British Museum of Natural History (Botany) 5: 291-355.
Robson N. K. B. 1981. Studies in the genus Hypericum L. (Guttiferae) 2. Characters of the genus. Bull. Br. Mus. nat. Hist. (Bot.) 8(2): 55-226.
Robson N. K. B. 1996. Studies in the genus Hypericum L. (Guttiferae) 6. Sections 20. Myriandra to 28. Elodes. Bull. not. Hist. Mus. Land. (Bot.) 26(2): 75-217.
Robson N. K. B. 2002. Studies in the genus Hypericum L. (Guttiferae) 4(2). Section 9. Hypericum sensu lato (part 2): subsection 1. Hypericum series 1. Hypericum. Bull. Nat. Hist. Mus. (London), Bot. 32: 61-123.
Robson N. K. B. 2010a. Studies in the genus Hypericum L. (Hypericaceae) 5(1). Sections 10. Olympia to 15/16. Crossophyllum. Phytotaxa 4: 5-126.
Robson N. K. B. 2010a. Studies in the genus Hypericum L (Hypericaceae) 5(2). Sections 17. Hirtella to 19. Coridium. Phytotaxa 4: 127-258.
StatSoft, Inc. 2013. Electronic Statistics Textbook. Tulsa, OK: StatSoft. WEB : http://www.statsoft.com/textbook/.
Stevens P. F 2007. Hypericaceae. In: K. Kubitzki (ed.). The Families and Genera of Vascular Plants, 9, pp. 194-201. Springer Verlag Berlin-Heidelberg.
Szkudlarz P. 2014. Seed morphology and anatomy of Hypericum elegans Steph. ex Willd. Biodiv. Res. Conserv. 35: 15-18.
Ward J. H. 1963. Hierarchical grouping to optimize an objective function. Journal of American Statistical Association 58(301): 236-244.